PASA allows generating quantitative and qualitative molecular measurements related to serum antibody repertoire from high-resolution proteomic data. PASA is applicable for researchers at all levels that are interested in addressing basic questions related to B cell development and differentiation, as well as an application focused tool for vaccine development, immunodiagnostic discovery and monoclonal antibody engineering. The web server accepts proteomics spectral files (in '.raw' format), and requires (at least) two technical replicates of each fraction (elution and flow-through). See running example input and output.


Peptides data type:

Elution raw data:

Flow-through raw data:

Please notice that since the raw files are (very) large. Thus, it might require a few hrs to upload the data, depending on your network speed. It is recommended to use Ethernet connection rather than WiFi.